Composite

Part:BBa_K575034:Design

Designed by: Kristin Palarz   Group: iGEM11_Northwestern   (2011-09-22)

RhlR/PAI2 Inducible Promoter + RBS (B0030) + RFP + Constitutive Promoter + RBS (B0030) + RhlR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 803
    Illegal NheI site found at 826
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1100
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 637
    Illegal AgeI site found at 749
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1575

Design Notes

The constitutive promoter is located downstream of the inducible promoter because the reverse alignment may have resulted in unwanted leaky transcription of GFP.

Source

The pieces of this construct are all existing registry parts.

References